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T-1824 or Evans blue, often incorrectly rendered as Evan's blue, is an azo dye that has a very high affinity for serum albumin. Because of this, it can be useful in physiology in estimating the proportion of body water contained in blood plasma. It fluoresces with excitation peaks at 470 and 540 nm and an emission peak at 680 nm.
22 de abr. de 2018 · Evans blue (EB) dye has owned a long history as a biological dye and diagnostic agent since its first staining application by Herbert McLean Evans in 1914.
- Linpeng Yao, Xing Xue, Peipei Yu, Yicheng Ni, Feng Chen
- 10.1155/2018/7628037
- 2018
- 2018
22 de abr. de 2018 · Evans blue (EB) dye has owned a long history as a biological dye and diagnostic agent since its first staining application by Herbert McLean Evans in 1914. Due to its high water solubility and slow excretion, as well as its tight binding to serum albumin, EB has been widely used in biomedicine, incl ….
- Linpeng Yao, Xing Xue, Peipei Yu, Yicheng Ni, Feng Chen
- 2018
29 de out. de 2015 · For example using a 2% compared to 0.5% solution of Evans blue there was about 70% more free dye at 6 h after injection. On examining the brains of mice injected with this higher concentration of Evans blue, the dye was detected intraneuronally at several sites in the brain.
- Norman R. Saunders, Katarzyna M. Dziegielewska, Kjeld Møllgård, Mark D. Habgood
- 10.3389/fnins.2015.00385
- 2015
- Front Neurosci. 2015; 9: 385.
8 de ago. de 2017 · We present an easy, quick, cost-effective, staining and spectrophotometric method to assess membrane stability of plant cells. In this method, Evan’s blue, an azo dye, is used to assay for cell viability. More specifically, Evan’s blue dye can penetrate through ruptured or destabilized membranes and stain cells.
- Preethi Vijayaraghavareddy, Vanitha Adhinarayanreddy, Ramu S Vemanna, Sheshshayee Sreeman, Udayakuma...
- 10.21769/BioProtoc.2519
- 2017
- Bio Protoc. 2017 Aug 20; 7(16): e2519.
Evans Blue dye is a vital dye that, when injected intravenously, binds avidly to plasma proteins. As such, it has been used as a marker for protein extravasation in models of altered microvascular permeability.
The agent most commonly used for acute studies of osmotic barrier opening, to assess BBB integrity, is Evans blue-albumin. Evans blue dye binds tightly but reversibly to albumin in vivo (48), resulting in an M r 68,500 marker that does not cross an intact BBB, but that will stain brain parenchyma following disruption (Fig. 4) and will provide a ...
