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A new microdilution method has been developed for determining the minimum inhibitory concentration (MIC) of oil-based compounds. The redox dye resazurin was used to determine the MIC of a sample of the essential oil of Melaleuca alternifolia (tea tree) for a range of Gram-positive and -negative bacteria.
- C.M. Mann, J.L. Markham
- 1998
6244/05/97: received 23 May 1997 and accepted 31 July 1997 C.M. MANN AND J.L. MARKHAM. 1998. A new microdilution method has been developed for determining the minimum inhibitory...
5 de jan. de 2002 · Citing Literature. Volume 84, Issue 4. May 1998. Pages 538-544. A new microdilution method has been developed for determining the minimum inhibitory concentration (MIC) of oil-based compounds. The redox dye resazurin was used to determine the MIC of a sample of...
Mann, C. M., & Markham, J. L. (1998). A new method for determining the minimum inhibitory concentration of essential oils. Journal of Applied Microbiology, 84 (4), 538–544. doi:10.1046/j.1365-2672.1998.00379.x
1 de mai. de 1998 · C. Mann, J. Markham. Published in Journal of Applied… 1 May 1998. Chemistry, Environmental Science, Medicine. TLDR. Use of 0·15% (w/v) agar as a stabilizer overcame the problem of adequate contact between the oil and the test bacteria and obviated the need to employ a chemical emulsifier. Expand. View on Wiley. ncbi.nlm.nih.gov. Save to Library.
O método de microdiluição proposto por Mann e Markham (1998) para a determinação a CIM de óleos essenciais baseou-se na comparação da leitura visual e fotométrica das microplacas e demonstrou que os resultados da microdiluição podem ser avaliados sem instrumentação.
Mann e Markham (1998) desenvolveraram um método de micro-diluição em caldo conveniente para determinar a atividade antimicrobiana dos óleos essenciais usando um estabilizador químico e microbiologicamente inerte (corante resazurina), que é um indicador capaz de demonstrar com segurança o CIM, tanto visualmente como instrumentalmente.
