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  1. Há 5 dias · Editing three loci simultaneously with 100 bp non-selectable homologous arms resulted in a 45% homologous recombination rate. These results suggest that efficient homologous recombination gene editing mediated by SSB/CRISPR-Cas9 can be achieved using donor DNA with 90–100 bp non-selectable homologous arms or 60–100 bp selectable ...

  2. Há 5 dias · The number of big genera has increased from 57 to 86; today one of every four plant species is classified as a member of a big genus, with 14% in just 28 megadiverse genera. Most (71%) of the growth in big genera since 2000 is the result of new species description, not generic re-circumscription.

  3. Há 1 dia · Neste contexto, investidores de renome como Warren Buffett, Ray Dalio e Peter Lynch promovem uma visão de longo prazo, fundamental também para o entendimento e sucesso no mercado brasileiro. Warren Buffett, um dos maiores investidores do mundo, defende que “O tempo no mercado é mais importante do que o timing do mercado”.

  4. Há 10 horas · Buffett comprou a casa de cinco quartos por US$ 31.500 em 1958, o que equivale a aproximadamente US$ 330 mil (R$ 1,7 milhões) no valor atual. “Estou feliz lá”, diz ele.

  5. Há 4 dias · @article{Pitiyarachchi2024HomologousRD, title={Homologous recombination deficiency should be tested for in patients with advanced stage high-grade serous ovarian cancer aged 70 years and over.}, author={Omali Pitiyarachchi and P.J. Ansell and Robert L. Coleman and Minh H. Dinh and Laura Holman and Charles A. Leath and Theresa Werner and Paul DiSilvestro and Mark Morgan and William Tew and ...

  6. Há 6 dias · An efficient recombination system for chromosome engineering in Escherichia coli. Mini-λ: a tractable system for chromosome and BAC engineering. Bacterial Artificial Chromosome Mutagenesis Using Recombineering. Rapid generation of markerless recombinant MVA vaccines by en passant recombineering of a self-excis...

  7. Há 2 dias · However, the linked Ros.El allele present in Sippe50 must have arisen through a recombination event between an Eluta allele from A. latifolium and a Ros + allele from A. majus. Such a combination was possibly selected for by horticulturalists for their stronger and higher contrast bullseye patterns that would appeal to home gardeners.