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Answering numerous requests for automatic Seed-Quality annotations for more or less complete bacterial and archaeal genomes, we have established the free RAST-Server (RAST=Rapid Annotation using Subsytems Technology). Using similar technology, we make the Metagenomics-RAST-Server freely available.
- RAST Tutorial
The RAST (Rapid Annotation using Subsystem Technology)...
- SEED Viewer Tutorial
This tool allows the user to identify protein functions that...
- RAST Tutorial
Annotating a Genome Using RAST. With RAST, it is now possible to get a fairly accurate annotation of a prokaryotic genome in about a day. We believe that the result is often very close to what most annotation groups can produce spending months or even man-years.
Besides producing initial assignments of gene function and a metabolic reconstruction, the RAST server provides an environment for browsing the annotated genome and comparing it to the hundreds of genomes maintained within the SEED integration.
- The Rast Server Overview
- Jobs Overview
- Reviewing Submitted/Completed Jobs
- Job Details
The RAST (Rapid Annotation using Subsystem Technology) Server provides high quality genome annotations for prokaryotes across the whole phylogenetic tree. It makes a SEED-quality annotation available as a service with a 48 hour turnaround time. The SEED environment and SEED data structures (most prominently FIGfams) are used to compute the automati...
Upon logging onto the server, users are directed to the “Jobs Overview” page, which as the name suggests, provides a site for job management. Jobs Overview has two main components: starting a new job and reviewing submitted/completed jobs. Start a new job. The navigation bar (Figure 2) at the top of the page provides a pull down menu for job submis...
The overall status of genome analysis can be viewed from the main portion of the Jobs Overview page. This contains information regarding a user’s personal jobs and when applicable, jobs of the user’s organization. Figure 3 shows an example account where the individual does not have any personal jobs, but has access to several for their organization...
For a given job, the Job Details page provides the user with information regarding the status of the genome annotation progress, as well as access to the results of the analysis upon completion. Account and job management links are found in the navigation bar at the top of the Job Details page (Figure 4) includes (1) logout, (2) upload a new genome...
RAST is a publicly-available server for the annotation of microbial genomes. It is maintained by a team at Argonne National Lab and FIG. Currently, it has over 2600 registered users, and several thousand genomes have been run through the service in the last couple of years (often several times!).
This tool allows the user to identify protein functions that are the same or different for a set of organisms. The results are presented in tabular form with information regarding function, EC and subsystem involvement displayed. Each protein is linked to the Annotation Overview in the SEED Viewer. 6.
RAST is a web-based environmet that allows users to upload a genome, annotate the genome, edit the annotations and compare the genome with other sequenced genomes in the SEED database.
