Resultado da Busca
4 de mar. de 2016 · Lentivirus-delivered CRISPR/Cas9 significantly diminished HIV-1 replication in infected primary CD4+ T-cell cultures and drastically reduced viral load in ex vivo culture of CD4+ T-cells...
- Rafal Kaminski, Yilan Chen, Tracy Fischer, Ellen Tedaldi, Alessandro Napoli, Yonggang Zhang, Jonatha...
- 2016
10 de nov. de 2021 · Both lentiviral and lipid nanoparticle (LNP) delivery of CRISPR-Cas9 HIV-1 excision therapies proved successful in blocking HIV-1 reactivation from latently infected CD4+ T and monocytes and primary human macrophages.
- Jonathan Herskovitz, Mahmudul Hasan, Milankumar Patel, Wilson R. Blomberg, Wilson R. Blomberg, Jacob...
- 2021
1 de dez. de 2022 · A CRISPR/Cas9 introduces double stranded breaks in the HIV-1 LTR and/or viral genes thereby inactivating the proviral genome. Use of multiple gRNAs results in excision or hypermutation of the target sites.
- Ruchira Bhowmik, Binay Chaubey
- AIDS Res Ther. 2022; 19: 58.
- 10.1186/s12981-022-00483-y
- 2022
22 de mar. de 2019 · Ebina et al. successfully used CRISPR/Cas9 to suppress the expression of HIV-1 genes in Jurkat cell lines by targeting HIV-1 LTR (Ebina et al., 2013). The target sites were the NF-κB binding cassettes located in the U3 region of LTR and TAR sequences in R region, respectively.
- Qiaoqiao Xiao, Qiaoqiao Xiao, Deyin Guo, Shuliang Chen, Shuliang Chen
- 10.3389/fcimb.2019.00069
- 2019
- Front Cell Infect Microbiol. 2019; 9: 69.
26 de ago. de 2013 · LTR-specific editing by CRISPR/Cas9 components disrupts HIV-1 expression machinery. We designed a gRNA expression vector to target HIV-1 LTR under the control of the human U6 polymerase III...
- Hirotaka Ebina, Naoko Misawa, Yuka Kanemura, Yoshio Koyanagi
- 2013
2 de jul. de 2019 · Elimination of HIV-1 requires clearance and removal of integrated proviral DNA from infected cells and tissues. Here, sequential long-acting slow-effective release antiviral therapy (LASER ART...
15 de set. de 2021 · It was the first demonstration that targeting of the HIV-1 LTR by CRISPR-Cas9 could block LTR-driven gene expression and lead to the excision of the region encompassed between the LTRs, with potential eradication of the provirus.
