Resultado da Busca
23 de jun. de 2020 · The results of the current study show efficient lentivirus transduction in the salmonid cell line CHSE-214 and the first demonstration of lentivirus-mediated editing in salmonid fish cells. The optimised protocol described results in the most efficient genome editing of a salmonid cell line to date.
- Remi L. Gratacap, Tim Regan, Carola E. Dehler, Samuel A. M. Martin, Pierre Boudinot, Bertrand Collet...
- 2020
12 de abr. de 2021 · CRISPR/Cas9 gene editing is effective in manipulating genetic loci in mammalian cell cultures and whole fish but efficient platforms applicable to fish cell lines are currently limited.
- Jens Hamar, Dietmar Kültz
- 2021
23 de nov. de 2023 · CRISPR/Cas9 HDR system was used to integrate exogenous alligator cathelicidin gene into targeted non-coding region of channel cat fish genome to acquire resistance against certain diseases (Simora et al. 2020).
25 de jul. de 2021 · The commercialization of the growth-enhanced AquAdvantage salmon and the CRISPR/Cas9-developed tilapia (Oreochromis niloticus) proffers genetic engineering and genome editing tools, e.g. CRISPR/Cas, as potential solutions to these challenges.
- Arinze S Okoli, Torill Blix, Anne I Myhr, Wenteng Xu, Xiaodong Xu
- 2021
14 de set. de 2022 · CRISPR FISHer enables high-sensitivity imaging of nonrepetitive DNA in living cells through phase separation-mediated signal amplification. Xin-Yuan Lyu, Yuan Deng, Xiao-Yan Huang,...
22 de set. de 2015 · Here we report a novel approach that uses in vitro constituted nuclease-deficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated caspase 9 (Cas9) complexes as probes to label sequence-specific genomic loci fluorescently without global DNA denaturation (Cas9-mediated fluorescence in situ ...
2 de fev. de 2022 · CRISPR-Cas9 genome editing has potential to cure diseases without current treatments, but therapies must be safe. Here we show that CRISPR-Cas9 editing can introduce unintended mutations in...
